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primary samples jurkat cells  (ATCC)


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    ATCC primary samples jurkat cells
    Primary Samples Jurkat Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 19570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+medium/RPMI-1640+Medium/pm42284413-241-3-10
    Average 99 stars, based on 19570 article reviews
    primary samples jurkat cells - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Characterization of a functional Ca 2+ toolkit in urine-derived stem cells and derived skeletal muscle cells.
    Article Snippet: Muscular diseases are characterized by a wide genetic diversity and the Ca2+-signalling machinery is often perturbed.. Its characterization is therefore pivotal and requires appropriate cellular models.. Muscle biopsies are the best approach but are invasive for the patient and difficult to justify if the biopsy is not for diagnostic purposes.

    Isolation:

    Article Title: ATM knock out alters calcium signalling and augments contraction in skeletal muscle cells differentiated from human urine-derived stem cells.
    Article Snippet: .. The samples were preserved with 10% primary medium (DMEM/ F12, Sigma Aldrich, Cat. #D8062; 10% FBS, Euroclone, Cat. #ECS5000L; 1% penicillin-streptomycin, Euroclone, Cat. #ECB3001; 2.5 μg/ml amphotericin B, GIBCO, Cat. #15290018; Renal Epithelial Growth Medium SingleQuot supplement, LGC Standard, Cat. # ATCC-PCS-400-040) for 1 h at 4 °C before the isolation. ..

    Article Title: ATM knock out alters calcium signalling and augments contraction in skeletal muscle cells differentiated from human urine-derived stem cells
    Article Snippet: .. The samples were preserved with 10% primary medium (DMEM/F12, Sigma Aldrich, Cat. #D8062; 10% FBS, Euroclone, Cat. #ECS5000L; 1% penicillin-streptomycin, Euroclone, Cat. #ECB3001; 2.5 μg/ml amphotericin B, GIBCO, Cat. #15290018; Renal Epithelial Growth Medium SingleQuot supplement, LGC Standard, Cat. # ATCC-PCS-400-040) for 1 h at 4 °C before the isolation. ..



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    Image Search Results


    (A) Venn diagram showing overlap between ATAC-seq summits and H3K27Ac peaks (±500 nt), used to define 134,520 active enhancers in PHHs. (B) Genomic classification of hepatocyte enhancers relative to position of protein-coding genes (GENCODE v43). (C) Heatmaps of PRO-seq (left), H3K27Ac ChIP-seq (center), and ATAC-seq (right) signal at active enhancers, centered on ATAC-seq summits (± 1,500 nt). Color scale represents normalized read coverage (RPM) per 10 nt bin. Signal above the dashed line corresponds to the 96,169 enhancers exhibiting detectable transcriptional activity, defined as >0.02 PRO-seq reads per million (RPM). (D) Histogram of PRO-seq signal intensities (RPM) for the top 10,000 paRNA– and eRNA-producing regions. PRO-seq signal was measured within a 1 kb window centered on ATAC-seq peak summits. Reads mapped to the plus and minus strands were quantified separately. For enhancers (not overlapping promoters), PRO-seq signal was measured within ±500 nt of ATAC-seq peak summits. For intragenic enhancers, only antisense signal relative to the direction of transcription of the corresponding host gene was quantified. PRO-seq signals for paRNAs were similarly quantified within ±500 nt of ATAC-seq peak summits located within ±1 kb of annotated TSSs using only antisense signal relative to gene transcription. Mean and median values are indicated. (E) Histogram showing PRO-seq read distribution for 13,979 paRNA– and 68,052 eRNA-producing loci. PRO-seq signals were calculated as in (D). Intragenic enhancers with only sense PRO-seq signal relative to the host gene transcription were excluded. Mean and median values are indicated.

    Journal: bioRxiv

    Article Title: Profiling and Targeting of Regulatory RNAs to Upregulate Gene Expression

    doi: 10.64898/2026.04.21.719874

    Figure Lengend Snippet: (A) Venn diagram showing overlap between ATAC-seq summits and H3K27Ac peaks (±500 nt), used to define 134,520 active enhancers in PHHs. (B) Genomic classification of hepatocyte enhancers relative to position of protein-coding genes (GENCODE v43). (C) Heatmaps of PRO-seq (left), H3K27Ac ChIP-seq (center), and ATAC-seq (right) signal at active enhancers, centered on ATAC-seq summits (± 1,500 nt). Color scale represents normalized read coverage (RPM) per 10 nt bin. Signal above the dashed line corresponds to the 96,169 enhancers exhibiting detectable transcriptional activity, defined as >0.02 PRO-seq reads per million (RPM). (D) Histogram of PRO-seq signal intensities (RPM) for the top 10,000 paRNA– and eRNA-producing regions. PRO-seq signal was measured within a 1 kb window centered on ATAC-seq peak summits. Reads mapped to the plus and minus strands were quantified separately. For enhancers (not overlapping promoters), PRO-seq signal was measured within ±500 nt of ATAC-seq peak summits. For intragenic enhancers, only antisense signal relative to the direction of transcription of the corresponding host gene was quantified. PRO-seq signals for paRNAs were similarly quantified within ±500 nt of ATAC-seq peak summits located within ±1 kb of annotated TSSs using only antisense signal relative to gene transcription. Mean and median values are indicated. (E) Histogram showing PRO-seq read distribution for 13,979 paRNA– and 68,052 eRNA-producing loci. PRO-seq signals were calculated as in (D). Intragenic enhancers with only sense PRO-seq signal relative to the host gene transcription were excluded. Mean and median values are indicated.

    Article Snippet: At 4 hours post-plating, cells were treated with 5 μM ASO via gymnosis in Cellartis Power Primary Hepatocyte Medium.

    Techniques: ChIP-sequencing, Activity Assay